Characterization of the cilia and ciliary membrane proteins of wild-type Paramecium tetraurelia and a pawn mutant.
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چکیده
منابع مشابه
Characterization of the cilia and ciliary membrane proteins of wild- type Paramecium tetraurelia and a pawn mutant
Cilia and ciliary membranes were isolated from axenically grown, wild-type Paramecium tetraurelia strain 51s and from the extreme pawn mutant strain, d495, derived from this parental strain. Over 60 protein bands having molecular weights of 15 to greater than 300 kdaltons were detected by Coomassie Blue staining of whole cilia proteins separated by one-dimensional SDS polyacrylamide gel electro...
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چکیده ندارد.
Proteomic analysis of the cilia membrane of Paramecium tetraurelia.
Channels, pumps, receptors, cyclases and other membrane proteins modulate the motility and sensory function of cilia, but these proteins are generally under-represented in proteomic analyses of cilia. Studies of these ciliary membrane proteins would benefit from a protocol to greatly enrich for integral and lipidated membrane proteins. We used LC-MS/MS to compare the proteomes of unfractionated...
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The swimming behavior of Paramecium is regulated by an excitable membrane that covers the body and cilia of the protozoan. In order to obtain information on the topology and function of ciliary membrane proteins, Paramecia were treated with trypsin, chymotrypsin or pronase and the effects of these proteases were analyzed using electron microscopy, gel electrophoresis of ciliary fractions and be...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1981
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.89.2.206